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KMID : 0352519970340010015
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Korea Univercity Medical Journal
1997 Volume.34 No. 1 p.15 ~ p.24
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Interaction of Calcitriol and Prednisolone on the Polyamine Metabolism, [14C] Spermine-binding Protein, and [3H] Thymidine DNA Uptake in the Rat Duodenal mucosa
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최상현
이영재/정휘정/김형건/신경호/천연숙/전보권
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Abstract
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Calcitriol has been shown to induce the duodenal synthesis of the spermine-binding protein, and hydrocortisone was reported to increase the intestinal polyamine contents.
This study examined whether prednisolone might enhance or reduce the calcitriol effects on the polyamine contents, [14C]spermine-protein binding activity, and [3H] thymidine DNA synthesis of the rat duodenum.
The mucosal contents of putrescine, spermidine, and spermine were increased by 198.8%m 84.0%, and 78.3%, respectively, within 6 hours after calcitriol, 5 ug/kg (CT) and fell down to the control values at 12 hours. And prednisolone, 10 mg/kg (PD)
also
increased the polyamine contents in similar patterns. They did not counteract each other in the polyamine metabolism.
CT increased the [14C] spermine-binding activity of the cytosol protein fraction from the duodenal mucosa by 71.1%, But PD increased the binding activity by 28.0% and did not affect the CT-induced increase.
CT increased the [3H] thymidine DNA synthesis of the duodenal mucosa by 42.3%, but PD reduced it slightly. The CT-dependent increase of the synthesis was significantly inhibited by PD.
These results suggested that the inhibitory effect of glucocorticoids on the vitamin D dependent increases of the duodenal calcium absorption and mucosal proliferation seems
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